Description
Interleukin-2 (IL-2) is a potent, multifunctional cytokine produced by activated T lymphocytes. It is responsible for the activities attributed to T cell growth factor, thymocyte stimulation factor, thymocyte mitogenesis factor, killer helper factor, and T cell replacing factor. IL-2 stimulates the growth and differentiation of T cells, as well as cytokine production by T cells. In addition, IL-2 can contribute to the activation of monocytes/macrophages and NK and LAK cells and can augment B cell growth and differentiation.
Formulation and Purity
Recombinant rat IL-2 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution (pH 7.2) and containing 2 mg/mlbiotechnology grade, low-endotoxin bovine serum albumin, glycerol and with no preservatives. The endotoxin level is ≤ 0.1 ng per μg of rat IL-2 protein, as measured in a LAL chromogenic assay. This recombinant rat IL-2 is a single band of approximately 16.5 kD as determined by SDS-PAGE analysis on 10 - 20% gels run under reducing conditions.
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Preparation and Storage
This preparation contains no preservatives, thus it should be handled under aseptic conditions.Store product at -80°C prior to use or for long term storage of stock solutions.Rapidly thaw and quick-spin product prior to use.Avoid multiple freeze-thaws of product.Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at –80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein, such as human or bovine albumin, aliquoted and stored at ≤ -70°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 – 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 10 μg/ml for long term storage. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
1. Biological Activity
Activity is tested: in a Proliferation assay using CTLL-2 indicator cells
Specific activity: 0.6 - 5.0 x 10^8 Units*/mg
ED50: 20 - 150 pg/ml
Observed Dose response: 100 fold
* A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.
2. ELISA Standard
Recombinant rat IL-2 is useful as a quantitative standard for measuring rat IL-2 protein levels in an IL-2 specific sandwich ELISA. To obtain linear standard curves, doubling dilutions of this rat IL-2 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.
Note: This application is not routinely tested at BD Biosciences.
