Description
Granulocyte macrophage colony stimulating factor (GM-CSF) is a potent species-specific stimulator of bone marrow cells. GM-CSF stimulates precursor cells of granulocytes, macrophages, and eosinophils. Human GM-CSF is a 14 - 17 kD multiple glycosylated protein containing 123 amino acid residues.Recombinant human GM-CSF (Cat. No. 550068) is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing bovine serum albumin with no preservatives.Recombinant human GM-CSF is≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of human GM-CSF, as measured in a chromogenic LAL assay.
Suggested Companion Products
Purified Rat Anti-Human GM-CSF BVD2-23B6RUO 0.5mgCat No: 554502
Biotin Rat Anti-Human GM-CSF BVD2-21C11RUO 0.5mgCat No: 554505
Human GM-CSF ELISA Set RUO 20Cat No: 555126
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Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions.Rapidly thaw and quick-spin product prior to use.Avoid multiple freeze-thaws of product.This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Upon initial thawing, recombinant human GM-CSF (Cat. No. 550068) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use.Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5-10 mg/mL carrier protein, such as human or bovine serum albumin, aliquoted and stored at -80°C.For in vitro biological assay use, carrier-protein concentrations of 0.5– 1 mg/mL are recommended.For use as an ELISA standard, carrier-protein concentrations of 5– 10 mg/mL are recommended.Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.The product should not be diluted to less than 5 μg/mL for long term storage.Carrier proteins should be pre-screened for possible effects in each investigator"s experimental system.Carrier proteins may have an undesired influence onexperimental results due to toxicity, high endotoxin levels or possible blocking activity.
ELISA Standard: Recombinant human GM-CSF (Cat. No. 550068) can be useful as a quantitative standard for measuring human GM-CSF protein levels using sandwich ELISA with the purified BVD2-23B6 antibody (Cat. No. 554502) as a capture antibody and biotinylated BVD2-21C11 (Cat. No. 554505) as the detection antibody.To obtain linear standard curves, investigators may want to consider using doubling dilutions of recombinant human GM-CSF from 2,000 - 15 pg/mL to be included for each ELISA plate.For measuring human GM-CSF in serum or plasma, investigators are highly encouraged to use the BD OptEIA™ Human GM-CSF ELISA Set (Cat. No. 555126).
Bioassay:Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments.An activity range of 0.1 - 1.0 x 10^9 units/mg, encompassing an
ED50= 10 - 90 pg/mL, has previously been reported using TF-1 as indicator cells for proliferation, with a unit defined as the amount of material needed to stimulate a half-maximal response at cytokine saturation.
Blocking: Recombinant human GM-CSF (Cat. No. 550068) can be useful as a blocking control for flow cytometric analysis when used with PE-conjugated BVD2-21C11 (Cat. No. 554507).Investigators are advised that the blocking application is not routinely tested for this material.Intracellular cytokine staining techniques and the use of blocking controls are described in detail by C. Prussin and D. Metcalfe.
