Description
Tumor Necrosis Factor (TNF, also known as TNF-α) is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells and certain other target cells. Human TNF is a 17.5 kD protein containing 157 amino acid residues.Recombinant human TNF (Cat. No. 554618) is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing bovine serum albumin, with no preservatives.Recombinant human TNF is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.The endotoxin level is ≤ 0.1 ng/μg of human TNF, as measured in a chromogenic LAL assay.
Suggested Companion Products
Purified Mouse Anti-Human TNF MAb1RUO 1mgCat No: 551220
Biotin Mouse Anti-Human TNF MAb11RUO 0.5mgCat No: 554511
Human TNF ELISA Set RUO 20Plate(s)Cat No: 555212
Human TNF ELISA Kit II RUO 2Plate(s)Cat No: 550610
Human TNF Flex Set RUO 100TestsCat No: 558273
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Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions.Rapidly thaw and quick-spin product prior to use.Avoid multiple freeze-thaws of product.This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Upon initial thawing, recombinant human TNF (Cat. No. 554618) should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5-10 mg/mL carrier protein, such as human or bovine albumin, aliquoted and stored at -80°C.For in vitro biological assay use, carrier-protein concentrations of≥ 1 mg/mL are recommended.For use as an ELISA standard, carrier-protein concentrations of 5-10 mg/mL are recommended.Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.Carrier proteins should be pre-screened for possible effects in each investigator"s experimental system.Carrier proteins may have an undesired influence on experimental results due to toxicity, high endotoxin levels or possible blocking activity.
ELISA Standard:Recombinant human TNF (Cat. No. 554618) can be useful as a quantitative standard for measuring human TNF protein levels using sandwich ELISA with the purified MAb1 antibody (Cat. No. 551220) as a capture antibody and biotinylated MAb11 antibody (Cat. No. 554511) as the detection antibody. To obtain linear standard curves, investigators may want to consider using doubling dilutions ofrecombinant human TNF standard from 2000-15 pg/mL to be included in each ELISA plate.For measuring human TNF in serum or plasma, investigators are highly encouraged to use BD OptEIA™ Human TNF ELISA Set (Cat. No. 555212) or BD OptEIA™ Human TNF ELISA Kit II (Cat. No. 550610).
Bioassay:Investigators are advised that the Bioassay application is not routinely tested for this material and are highly encouraged to both titrate this material and include appropriate controls in relevant experiments. Anactivity range of 0.05 - 2.0 x 10^9 units/mg, encompassing an
ED50= 5-200 pg/mL, has previously been reported using L929 as indicator cells in a cytolysis assay, with a unit defined as the amount of material needed to stimulate a half-maximal response at cytokine saturation.
