Description
Interleukin-1(IL-1β) is a potent immunomodulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells.Mouse IL-1is a 17 kD protein containing 153 amino acid residues.
Formulation and Purity: Recombinant mouse IL-1βis supplied lyophilized from a solution comprised of 0.22µm sterile-filtered aqueous buffered solution, pH 7.4, and containing 50 µg bovine serum albumin, per µg of cytokine.The product contains no preservatives. Recombinant mouse Interleukin-1βis > 95% pure as determined by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.
Suggested Companion Products
Mouse IL-1β ELISA Set RUO 20Cat No: 559603
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Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions.Avoid multiple freeze-thaws of product.The product can be reconstituted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C.For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml.For use as an ELISA standard we recommend carrier-protein concentrations of 5-10 mg/ml.Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
1.Biological Activity:As measured by proliferation ofTF-1 indicator cells at 10^5 cells/ml
Specific Activity:0.06 - 2.5 x10^8 Unit*/mg
ED50: 0.04-1.5 ng/ml; Observed dose response relationship: >50 fold
*Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation. Investigators are advised to determine optimal concentrations for individual applications.
2. ELISA Standard:.
Recombinant mouse IL-1β is useful as a quantitative standard for measuring mouse IL-1βprotein levels in an IL-1β -specific sandwich ELISA. For specific methodology, please visit the online protocols under "ELISA and ELISPOT" at our website, http://www.bdbiosciences.com/us/s/resources. To measure mouse IL-1βin plasma or serum, we recommend the BD OptEIA™ Mouse IL-1βELISA Set (Cat. No. 559603), which is specially formulated for assay of cytokine in complex fluids.
