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当前位置: 首页 > 产品中心 > peptide > BD Biosciences/BrdU In-Situ Detection Kit II/551321
商品详细BD Biosciences/BrdU In-Situ Detection Kit II/551321
BD Biosciences/BrdU In-Situ Detection Kit II/551321
BD Biosciences/BrdU In-Situ Detection Kit II/551321
商品编号: 551321
品牌: bdbiosciences
市场价: ¥18585.00
美元价: 10620.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Product DetailsRecommended AssayReferences
Preparation and Storage

All the components of the BrdU kit must be stored at 4°C. Some solutions require diluting or mixing prior to use.

  1. . Dolbeare, F., H. Gratzner, M. G. Pallavicini and J. W. Gray. 1983. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc. Natl. Acad. Sci. USA. 80:5573-5577.

  2. deFazio A., Leary J. A., Hedley D. W., and Tattersall M. H. 1987. Immunohistochemical detection of proliferating cells in vivo. J Histochem cytochem 35(5):571-577

  3. Dover R. and Patel K. 1994. Improved methodology for detecting bromodeoxyuridine in cultured cells and tissue sections by immunocytochemistry. Histochemistry 102(5):383-387.

  4. Ezaki T. 2000. Antigen retrieval on formaldehyde-fixed paraffin sections: its potential drawbacks and optimization for double immunostaining. Micron. 31(6)639-649.

  5. Fukuta K., Iwasaka T., Hachisuga T., Sugimori H., Tsugitomi H., Mutoh F. 1990. Immunocytochemical detection of S-phase cells in normal and neoplastic cervical epithelium by anti-BrdU monoclonal antibody. Anal Quant Cytol Histol 12(2):135-138

  6. Gratzner H.G. 1982. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science. 218:474-475.

  7. Holm, M., M. Thomsen, M. Høyer and P. Hokland. 1998. Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells. Cytometry. 32:28-36.

  8. Hume W. J. 1990. A reproducible technique combining tritiated thymidine autoradiography with immunodetection of bromodeoxyuridine for double labeling studies of cell proliferation in paraffin sections of tissues. Cell Tissue Kinet 23(3):161-168.

  9. Mckinley J. N., Knott K. K., and Thompson H. J. 2000. Effect of fixation and epitope retrieval on BrdU indices in mammary carcinomas. J Histochem cytochem 48(3):355-362.

  10. Morstyn G., Pyke K., Gardner J., Ashcroft R., de Fazio A., and Bhathal p. 1986. Immunohistochemical identification of proliferating cells in organ culture using bromodeoxyuridine and a monoclonal antibody. J Hictochem Cytochem 34(6):697-701.

  11. Qin Y. and Willems G. 1993. Comparison of the classical autoradiographic and the immunohistochemical methods with BrdU for measuring proliferation parameters in colon cancer. Anticancer Res 13(3):731-735

  12. Raza A., Ucar K., Bhayana R., Kempski M., and Preisler H.L. 1985. Utility and sensitivity of anti-BrdU antibodies in assessing S-phase cells compared to autoradiography. Cell Biochem funct 3(2):149-153.

  13. Robertson H., Wheeler J., and Morley A. R. 1990. In vivo bromodeoxyuridine incorporation in normal mouse kidney: immunohistochemical detection and measurement of labeling indices. Histochem J 22(4):209-214.

  14. Rocha, B., C. Penit, C. Baron, F. Vasseur, N. Dautigny, and A. A. Freitas. 1990. Accumulation of bromodeoxyuridine-labeled cells in central and peripheral lymphoid organs: minimal estimates of production and turnover rates of mature lymphocytes. Eur. J. Immunol. 20:1697-1708.

  15. Sasaki K., Ogino T., and Takahashi M. 1986. Immunological determination of labeling index on human tumor tissue sections using monoclonal anti-BrdU antibody. Stain Technol 61(3):155-161.

  16. Schutte B., Reynders M. M., Bosman F. T., and Blijham G. H. 1987. Studies with antibromodeoxyuridine antibodies: II. Simultaneous immunocytchemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa. J Histochem cytochem 35(3):371-374.

  17. Thoman, M. L. 1997. Early steps in T cell development are affected by aging. Cell. Immunol. 178:117-123.

  18. Tischler A. S. 1995. Triple immunohistochemical staining for bromodeoxyuridine and catecholamine biosynthetic enzymes using microwave antigen retrieval. J Histochem cytochem 43(1):1-4.

  19. Tough, D. F., and J. Sprent. 1994. Turnover of naive- and memory-phenotype T cells. J. Exp. Med. 179:1127-1135.

  20. von Boehmer, H., and K. Hafen. 1993. The life span of naive alpha/beta T cells in secondary lymphoid organs. J. Exp. Med. 177:891-896.

  21. Yu C. C., Woods A. L., and Levison D. A. 1992. The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications. Histochem J. 24 (3):121-131

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