4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:医疗设备、医疗耗材、实验仪器、抗体、试剂、诊断等产品
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > peptide > BD Biosciences/BrdU In-Situ Detection Kit/50Tests/550803
商品详细BD Biosciences/BrdU In-Situ Detection Kit/50Tests/550803
BD Biosciences/BrdU In-Situ Detection Kit/50Tests/550803
BD Biosciences/BrdU In-Situ Detection Kit/50Tests/550803
商品编号: 550803
品牌: bdbiosciences
市场价: ¥7560.00
美元价: 4320.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Product DetailsRecommended AssayReferences

Description

The BD Pharmingen™ BrdU In-Situ Detection Kit is designed for immunohistochemical staining of BrdU in frozen sections, formalin-fixed paraffin-embedded sections, and cultured or isolated cells on slides. Our monoclonal antibody against BrdU works in all species tested (human, mouse, and rat) and provides improved specific staining with minimal background. The directly biotinylated mouse antibody eliminates the need for a species-specific secondary antibody thus allowing it to be used in mouse tissues. The BrdU In-Situ Detection Kit features our BD™ Retrievagen A, an antigen retrieval solution designed specifically to unmask antigenic sites, preserve tissue morphology, and enable simultaneous staining of other surface antigens in conjunction with BrdU. This important feature of the kit enables study of the proliferation state of phenotypically defined cells within the micro-environment of tissues. Consistent results are assured by providing all critical reagents in addition to a comprehensive series of protocols for staining BrdU in paraffin-embedded and frozen tissue sections and cultured cells, and in conjunction with other antigens. Control slides are provided in the kit to serve as a reference.

Preparation and Storage

All the components of the BrdU kit must be stored at 4°C. Some solutions require diluting or mixing prior to use.

  1. . Dolbeare, F., H. Gratzner, M. G. Pallavicini and J. W. Gray. 1983. Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc. Natl. Acad. Sci. USA. 80:5573-5577.

  2. deFazio A., Leary J. A., Hedley D. W., and Tattersall M. H. 1987. Immunohistochemical detection of proliferating cells in vivo. J Histochem cytochem 35(5):571-577

  3. Dover R. and Patel K. 1994. Improved methodology for detecting bromodeoxyuridine in cultured cells and tissue sections by immunocytochemistry. Histochemistry 102(5):383-387.

  4. Ezaki T. 2000. Antigen retrieval on formaldehyde-fixed paraffin sections: its potential drawbacks and optimization for double immunostaining. Micron. 31(6)639-649.

  5. Fukuta K., Iwasaka T., Hachisuga T., Sugimori H., Tsugitomi H., Mutoh F. 1990. Immunocytochemical detection of S-phase cells in normal and neoplastic cervical epithelium by anti-BrdU monoclonal antibody. Anal Quant Cytol Histol 12(2):135-138

  6. Gratzner H.G. 1982. Monoclonal antibody to 5-bromo and 5-iododeoxyuridine: a new reagent for detection of DNA replication. Science. 218:474-475.

  7. Holm, M., M. Thomsen, M. Høyer and P. Hokland. 1998. Optimization of a flow cytometric method for the simultaneous measurement of cell surface antigen, DNA content, and in vitro BrdUrd incorporation into normal and malignant hematopoietic cells. Cytometry. 32:28-36.

  8. Hume W. J. 1990. A reproducible technique combining tritiated thymidine autoradiography with immunodetection of bromodeoxyuridine for double labeling studies of cell proliferation in paraffin sections of tissues. Cell Tissue Kinet 23(3):161-168.

  9. Mckinley J. N., Knott K. K., and Thompson H. J. 2000. Effect of fixation and epitope retrieval on BrdU indices in mammary carcinomas. J Histochem cytochem 48(3):355-362.

  10. Morstyn G., Pyke K., Gardner J., Ashcroft R., de Fazio A., and Bhathal p. 1986. Immunohistochemical identification of proliferating cells in organ culture using bromodeoxyuridine and a monoclonal antibody. J Hictochem Cytochem 34(6):697-701.

  11. Qin Y. and Willems G. 1993. Comparison of the classical autoradiographic and the immunohistochemical methods with BrdU for measuring proliferation parameters in colon cancer. Anticancer Res 13(3):731-735

  12. Raza A., Ucar K., Bhayana R., Kempski M., and Preisler H.L. 1985. Utility and sensitivity of anti-BrdU antibodies in assessing S-phase cells compared to autoradiography. Cell Biochem funct 3(2):149-153.

  13. Robertson H., Wheeler J., and Morley A. R. 1990. In vivo bromodeoxyuridine incorporation in normal mouse kidney: immunohistochemical detection and measurement of labeling indices. Histochem J 22(4):209-214.

  14. Rocha, B., C. Penit, C. Baron, F. Vasseur, N. Dautigny, and A. A. Freitas. 1990. Accumulation of bromodeoxyuridine-labeled cells in central and peripheral lymphoid organs: minimal estimates of production and turnover rates of mature lymphocytes. Eur. J. Immunol. 20:1697-1708.

  15. Sasaki K., Ogino T., and Takahashi M. 1986. Immunological determination of labeling index on human tumor tissue sections using monoclonal anti-BrdU antibody. Stain Technol 61(3):155-161.

  16. Schutte B., Reynders M. M., Bosman F. T., and Blijham G. H. 1987. Studies with antibromodeoxyuridine antibodies: II. Simultaneous immunocytchemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa. J Histochem cytochem 35(3):371-374.

  17. Thoman, M. L. 1997. Early steps in T cell development are affected by aging. Cell. Immunol. 178:117-123.

  18. Tischler A. S. 1995. Triple immunohistochemical staining for bromodeoxyuridine and catecholamine biosynthetic enzymes using microwave antigen retrieval. J Histochem cytochem 43(1):1-4.

  19. Tough, D. F., and J. Sprent. 1994. Turnover of naive- and memory-phenotype T cells. J. Exp. Med. 179:1127-1135.

  20. von Boehmer, H., and K. Hafen. 1993. The life span of naive alpha/beta T cells in secondary lymphoid organs. J. Exp. Med. 177:891-896.

  21. Yu C. C., Woods A. L., and Levison D. A. 1992. The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications. Histochem J. 24 (3):121-131

品牌介绍
BD是世界上最大的生产和销售医疗设备、医疗系统和试剂的医疗技术公司之一。致力于提高全世界人类的健康水平。BD专注于改进药物治疗,提高传染性疾病诊断的质量和速度,推进新型药物和疫苗的研究与发现。BD公司具有强大的研发能力和世界上最棘手的多种疾病进行斗争的能力。公司于1897年在纽约成立,总部位于美国新泽西州的富兰克林湖,业务遍及全球。公司的业务可分为BD医疗、BD诊断、BD生物科学三大类,生产销售包括医用耗材、实验室仪器、抗体、试剂、诊断等产品。公司的服务对象包括医疗机构,生命科学研究所,临床实验室,工业单位和普通大众。BD has state-of-the-art facilities around the globe that provide an environment that enables our highly talented workforce to be the best at their professions. We hire associates who have a passion and commitment to advancing the world of health. We are always seeking great people to join our company on its journey to greatness.BDRat Granulocytes Pure RP-1  500ug550002BDMs Ig Kpa Lgt Chain FITC 187.1  500ug550007BDICC Fixation Buffer 100mL550011BDMs CD210 NALE 1B1.3a 500ug550019BDMs CD51 Pure RMV-7 100ug550026BDMs CD5 APC 53-7.3 100ug550037BDHu CD62E PE-Cy5 68-5H11 100Tst550041BDHu CD95 Pure EOS9.1  100ug550052BDHam IgG2 Kpa ItCl FITC B81-3 250ug550057BDMs Crry/p65 Pure 1F2 100ug550059BDMs IL-4 Recom Protein 10ug550068BDMs IL-2 Recom Protein 20ug550070BDHu IL-6 Recom Protein 10ug550072BDHu MIP-1 Bta PE D21-1351 100ug550082BDMs IgG1 PE A85-1 100ug550084BDHam IgG2 Kpa ItCl PE B81-3 100ug550086BDHu CD142 Pure HTF-1 100ug550254BDHu CD1d PE CD1d42 100Tst550256BDHu CD73 PE AD2 100Tst550258BDHu CD103 FITC Ber-ACT8 100Tst550260BDMs CD11c APC HL3 100ug550268BDMs CD72b/c Aloatg PE JY/93 100ug550270BDRat CD32 Pure D34-485 500ug550272BDRat CD32 NALE D34-485 500ug550274BDMs CD3e IHC Pure 145-2C11 1mL550277BDMs CD4 IHC Pure H129.19 1mL550280BDMs CD8a IHC Pure 53-6.7 1mL
联络我们
服务热线:4000-520-616
(限工作日9:00-18:00)
QQ :1570468124
手机:18915418616