Description
Members of the caspase family play key roles in inflammation and mammalian apoptosis. Z-FA-FMK is a negative control inhibitor which has no inhibitory effect on apoptosis mediated by caspases and can only inhibit cysteine proteases (those not requiring P1 Asp inhibitors) such as Cathepsin B. Z-FA-FMK has a molecular weight of 386 Daltons.
Format
- FormatPurified
Suggested Companion Products
PE Annexin V Apoptosis Detection Kit I RUO Cat No: 559763
Z-VAD-FMK, General Caspase Inhibitor RUO 1mgCat No: 550377
Z-DEVD-FMK, Caspase-3 Inhibitor RUO 1mgCat No: 550378
Z-LEHD-FMK, Caspase-9 Inhibitor RUO 1mgCat No: 550381
Z-IETD-FMK, Caspase-8 Inhibitor RUO 1mgCat No: 550380
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| SpectrumViewer | Download TDS | Regulatory Document Website | ||||
Preparation and Storage
Avoid multiple freeze-thaws of product.Store lyophilized Z-FA-FMK at -20˚C. Reconstitute Z-FA-FMK in DMSO before use. Reconstituted Z-FA-FMK may be stored in small aliquots at -20˚C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
Z-FA-FMK is designed to be used in both in vivo and in vitro cell based assays as a negative control in assays used to measure apoptosis. Reconstitute 1.0 mg of Z-FA-FMK in DMSO. A 10 mM stock solution may be made by dissolving 1.0 mg of Z-FA-FMK in 263 µl DMSO. The final concentration of Z-FA-FMK needed may vary between experimental systems and investigators are encouraged to titrate. As a precautionary note, please do not exceed a final DMSO concentration of 0.2% as higher levels may cause cellular toxicity and mask the effects of the caspase inhibitor.
