Description

The human chromosomal region 11p15 has undergone intense analysis because of its association with various malignancies. In particular, the band 11p15.5 contains genes associated with Wilms tumor, Beckwith-Weidemann syndrome, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. One such gene, GOK (Stim 1), was identified near the 5" end of the ribonucleotide reductase subunit 1 gene. Examination of the GOK primary amino acid sequence indicates that it is a typical transmembrane protein with an extracellular N-terminal domain and a cytosolic C-terminal domain. The protein is highly hydrophobic with only a short region of hydrophobicity that likely represents the transmembrane region. The C-terminal portion of GOK shares some small regions of homology with myosin (20% identity). This region of GOK consists of á-helices and is thought to adopt a coiled-coil conformation. Although GOK expression has no effect on the growth of certain breast cancer cell lines, it induces death in rhabdomyosarcoma cells. Thus, it is thought to be a recessive tumor suppressor in muscle cells, possibly by functioning as a receptor connected to an apoptotic signaling pathway.

Format

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Preparation and Storage

Store undiluted at -20°C.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.