Description
BD Tritest™ Control, γ1 fluorescein isothiocyanate (FITC)/γ1 phycoerythrin (PE)/CD45 peridinin chlorophyll (PerCP), is a three-color direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with BD Tritest reagents on the BD family of flow cytometers. The BD Tritest Control is used to determine the unstained lymphocyte population on a fluorescence 1 (FL1) vs fluorescence 2 (FL2) display and to determine any nonantigen-specific antibody binding (nonspecific staining) present, particularly that caused by Fc receptors.
BD Tritest Control is for in vitro diagnostic use with BD in vitro diagnostic BD Tritest reagents.
Contents
| Specificity | Clone | Format | Isotype | Entrez Gene ID |
|---|---|---|---|---|
| X40 | FITC | IgG1, κ | ||
| X40 | PE | IgG1, κ | ||
| CD45 | 2D1 | PerCP | IgG1, κ |
| Resources & Tools | ||||||
|---|---|---|---|---|---|---|
| SpectrumViewer | Download MSDS | Download TDS | ||||
Preparation and Storage
• For in vitro diagnostic use.
• Store the reagent at 2°C–8°C to maintain stability. Do not use after expiration date shown on the label.
• Do not freeze the reagent or expose it to direct light during storage or during incubation with cells. Keep the reagent vial dry.
• Do not use the reagent if you observe any changes in appearance. Precipitation or discoloration indicates instability or deterioration.
• The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.
• All biological specimens and materials coming into contact with them are considered biohazards. Handle as if capable of transmitting infection and dispose of with proper precautions in accordance with federal, state, and local regulations when disposing of all materials. Never pipette by mouth. Wear suitable protective clothing, eyewear, and gloves.
• BD FACS lysing solution is required and contains diethylene glycol and formaldehyde. Refer to the BD FACS Lysing Solution instructions for use (IFU) for warnings.
