Description
BD™ Multi-Check Control is intended as a complete process control for immunophenotyping by flow cytometry. It is a control for antibody staining, red blood cell (RBC) lysis, instrument setup and performance, and data analysis on BD FACSLyric™, BD FACSCanto™ II, BD FACSCanto™, and BD FACSCalibur™ flow cytometers.
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Preparation and Storage
•Store vials upright, tightly capped, at 2°C–8°C when not in use.
• Unopened vials are stable until the expiration date indicated on each vial and Assay Values sheet.
• Opened vials are stable for 9 thermal cycles (uses) when handled properly. A thermal cycle constitutes performing all steps under Instructions for Use.
• Avoid unnecessary cycles of warming and cooling. Protect from freezing, from temperatures above 30°C, and from prolonged time (>30 minutes) at room temperature (20°C–25°C). Follow exactly the steps underInstructions for Use.
- Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline—Second Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2007. CLSI document H42-A2.
- Centers for Disease Control. 1997 Revisedguidelines for performing CD4+ T-celldeterminations in persons infected with humanimmunodeficiency virus (HIV). MMWR. 1997;46:1-29.
- Stelzer GT, Marti G, Hurley A, McCoy P Jr,Lovett EJ, Schwartz A. US-Canadian Consensusrecommendations on the immunophenotypicanalysis of hematologic neoplasia by flowcytometry: standardization and validation oflaboratory procedures. Cytometry. 1997;30:214-230.
