Description
The BD™ Stem Cell Enumeration (SCE) kit provides simultaneous enumeration of viable dual-positive CD45+/CD34+ hematopoietic stem cell populations in CD34+ absolute counts (cells/µL) as well as the percentage of the total viable leucocyte count that is CD34+ (%CD34). The following specimens can be analyzed with this kit: normal and mobilized peripheral blood, fresh and thawed leucopheresis products, fresh and thawed bone marrow, and fresh and thawed cord blood. The kit is intended for in vitro diagnostic (IVD) use on either a BD FACSCalibur™ flow cytometer using BD CellQuest™ or BD CellQuest™ Pro software or a BD FACSCanto™ II flow cytometer using BD FACSCanto™ software.
Contents: BD Stem Cell reagent (CD45 FITC/CD34 PE), 7-aminoactinomycin-D (7-AAD) reagent, 10X ammonium chloride lysing solution, 50 BD Trucount tubes.
| Resources & Tools | ||||||
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| SpectrumViewer | Download MSDS | IFU | Download TDS | Data Sheet | ||
Preparation and Storage
• Store the BD Stem Cell (CD45/CD34) reagent at 2°C to 8°C. Do not use after the expiration date shown on the label. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry.
• Store BD Trucount tubes in their original foil pouch at 2°C to 25°C. To avoid potential condensation, open the pouch only after it has reached room temperature and carefully reseal the pouch immediately after removing a tube. Examine the desiccant each time you open the pouch. If the desiccant has turned from blue to lavender, discard the remaining tubes. An unopened pouch is stable until the expiration date shown on the packaging. Do not open and use tubes after this expiration date. Use tubes within 1 hour after removal from the foil pouch. Use remaining tubes within 1 month after opening the pouch.
• Store 7-AAD at 2°C to 8°C. Protect from light.
• Store 10X ammonium chloride at 2°C to 8°C.
